TEST DIRECTORY | MIDx Labs

TREPONEMAL PALLIDUM (SYPH) Ab SCREEN

Detects antibodies to Treponema pallidum, used to screen for current or past syphilis infection.

LIS TEST CODE: SYPH

CPT CODE: 86592

ALIASES: SYPH, Treponema pallidum

METHODOLOGY: Chemiluminescent Microparticle Immunoassay (CMIA)

SPECIMEN:

- Type: Serum

- Container: Gold Top (SST) Vacutainer

- Minimum Volume Required: 2 mL

- Storage/Handling: Allow 30 minutes for blood to clot, then centrifuge specimen at 3500 RPM for 10 minutes. Maintain specimen at room temperature, in the refrigerator, or in the freezer until testing can be performed.

NOTE: If freezing is required to preserve stability, transfer serum into a separate plastic screw-cap transport container.

- Stability: Room Temperature (20-25 °C): 3 days

Refrigerated (2-8 °C): 7 days

Frozen (-20 °C or colder): 1 month; avoid multiple freeze/thaw cycles.

- Turnaround Time: 24 - 72 hours from receipt.

Special Instructions: Specimens that are initially reactive will be reflexed to a non-treponemal diagnostic test (RPR Screen with Reflex to Titer) for confirmation (CDC Reverse Algorithm).

If testing will be delayed for more than 7 days, remove serum from the separator gel and transfer into a separate plastic screw-cap transport container.

REJECTION REASONS: QNS, Gross Hemolysis, Gross Lipemia, Contamination, Stability Violation, Expired Container, Incorrect Container

REFERENCE RANGES:

Males & Females

>= 1 years of age: Nonreactive