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ALANINE AMINOTRANSFERASE (ALT)

Liver enzyme used to detect hepatocellular injury.

LIS TEST CODE: ALT

CPT CODE: 84460

ALIASES: ALT, GPT, SGPT

METHODOLOGY: NADH (without P-5'-P)

SPECIMEN:

- Type: Serum

- Container: Gold Top (SST) Vacutainer

- Minimum Volume Required: 1 mL

- Storage/Handling: Allow 30 minutes for blood to clot, then centrifuge specimen at 3500 RPM for 10 minutesMaintain specimen at either room temperature, in the refrigerator, or in the freezer until testing can be performed.

 

NOTE: If freezing is required to preserve stability, transfer serum into a separate plastic screw-cap transport container.

- Stability:     Room Temperature (20-25 °C): 3 days         

 

Refrigerated (2-8 °C): 7 days

                        Frozen (-20 °C or colder): 2 months; avoid multiple freeze/thaw cycles.

- Turnaround Time: 24 hours from receipt.

Special Instructions: N/A

REJECTION REASONS: QNS, Gross Hemolysis, Gross Lipemia, Contamination, Stability Violation, Expired Container, Incorrect Container

REFERENCE RANGES:

Males (U/L)

>= 1 year of age: 7 - 55

​​

Females (U/L)

>= 1 year of age: 7 - 45

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